Yes, but it won't work with all PS2 games. Most FPS titles will work with a mouse and keyboard and there may be a few others but those are the main type of games that this is used for. That's one good thing about having USB ports on the PS2 and also it has an IEEE port as well....better known as Firewire which will come in handy for connecting digital cameras to copy pics to the HDD whenever that's released. Well, i hope that you enjoy posting and have fun gaming.